Chromatography is one of the best separation methods. To create a column to conduct chromatography, the materials required are as follows: reaction mixture, silica, separatory funnel, 1:1 ethyl acetate/dichloromethane solvent, chromatography column, 15ml test tubes, test tube rack, plastic pipettes, and glass wool. In the chromatography column, a “wad” of glass wool was placed at the bottom of the column in order to stop the stop the silica that will be placed next from falling through. Then, the column was filled ¼ full with silica. With all the test tubes in the rack and ready, the reaction mixture was slowly poured in. Immediately followed by the reaction mixture, a 1:1 ethyl acetate/dichloromethane solvent allowed in by a separatory funnel. Slowly, the solvent started dripping down the column and into the test tubes. Once all 20 test tubes were filled up with ~3 ml of solution, the column was allowed to drip into a round bottom flask for the excess solution. Along with the 9A standard and 6TP standard, a drop was placed from each tube’s solution onto a 2x4 silica TLC plate (each plate had 5 drops each evenly spaced out). Next, the developing jar was filled up ½ cm with the 1:1 ethyl acetate/dichloromethane solvent. The TLC plate was then placed up against the side of the developing jar and the solvent was then allowed to rise up the TLC plate. Once the solvent has risen until there is only a centimeter left that is not damp with solvent. Lastly, the TLC plate was taken out and placed to dry and later be recorded. This process was repeated for all of the TLC plates. The reason as for why the TLC plates were redone is to pinpoint in which test tubes there was purified reacted substance.