To complete Proton NMR, the following materials were required; NMR tube, deuterated dimethyl sulfoxide (DMSO d-6), and 400 MHz NMR. After checking out all the TLC plates, it was determined which tubes consisted of the purified reacted substance. All of the tubes were then poured into a round bottom flask. Using deuterated dimethyl sulfoxide (DMSO d-6) as the NMR solvent, the 6TP standard, 9A standard and the newly created solution were separately mixed and put in separate NMR tubes. Then each sample was put in the 400 MHz NMR. The NMR experiments that were ran concluded that there was no starting material left in the tubes that were chosen because some of the peaks didn’t turn up as shown in Figure 6.
Figure 6. - 6TP Standard, 9A Standard, and Reaction Mixture Spectrums